Cytokeratin 19 Antikörper

Produktnummer ABIN94288

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cytokeratin 19 in WB, IHC (p), ICC, IP und ICFC. Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für Cytokeratin 19 Antikörper (ABIN94288)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Klon: BA-17

Produktdetails anti-Cytokeratin 19 Antikörper

Verwendungszweck: Anti-Cytokeratin 19 Purified

Spezifität: The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Mammary organoids.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Cytokeratin 19 Antikörper (ABIN94288)

Samluk, Zakrzewska, Pluta: "Generation of Fluorescently Labeled Cell Lines, C3A Hepatoma Cells, and Human Adult Skin Fibroblasts to Study Coculture Models." in: Artificial organs, (2013) (PubMed).

Lewis, Klimstra, Varmus: "The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer." in: Genes & development, Vol. 17, Issue 24, pp. 3127-38, (2003) (PubMed).

Bartek, Taylor-Papadimitriou, Miller, Millis: "Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 299-306, (1985) (PubMed).

Bartek, Durban, Hallowes, Taylor-Papadimitriou: "A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins." in: Journal of cell science, Vol. 75, pp. 17-33, (1985) (PubMed).

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: Cytokeratin 19

Hintergrund: Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gen-ID: 3880

UniProt: P08727