CD56 Antikörper

Produktnummer ABIN94187

Der Maus Monoklonal Anti-CD56-Antikörper wurde für FACS, IP, IHC (p) und ICC validiert. Er ist geeignet, CD56 in Proben von Human und Non-Human Primate zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für CD56 Antikörper (ABIN94187)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: MEM-188

Produktdetails anti-CD56 Antikörper

Verwendungszweck: Anti-Hu CD56 Purified

Spezifität: The antibody MEM-188 reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: KG-1 human acute myelogenous leukemia cell line

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 3 μg/mL. Immunohistochemistry (paraffin sections): Recommended dilution: 1:50, pretreatment: heat retrieval (microwave oven) in citrate buffer, standard ABC visualization, positive tissue: membrane-specific reaction in adjacent peripheral nerve, staining of paraprostatic autonomic ganglion.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD56 Antikörper (ABIN94187)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Olsen, Tollefsen, Aagaard, Reitan, Bannantine, Andersen, Sollid, Lundin: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, (2009) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: CD56

Hintergrund: Neural cell adhesion molecule 1,CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.,NCAM1, MSK39

Gen-ID: 4684

UniProt: P13591