ICAM1 Antikörper

Produktnummer ABIN94177

Dieser Maus Monoklonal Antikörper detektiert spezifisch ICAM1 in WB, FACS, ELISA, ICC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Rind (Kuh). Es wurde in 7+ Publikationen zitiert.

Kurzübersicht für ICAM1 Antikörper (ABIN94177)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-111

Produktdetails anti-ICAM1 Antikörper

Verwendungszweck: Anti-Hu CD54 Purified

Kreuzreaktivität (Details): Rat, Human, Bovine

Produktmerkmale: The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation, it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Raji human Burkitt's lymphoma cell line

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 0.5-4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus, RE cells.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-ICAM1 Antikörper (ABIN94177)

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, (2007) (PubMed).

Burdick, McCaffery, Kim, Bochner, Konstantopoulos: "Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow." in: American journal of physiology. Cell physiology, Vol. 284, Issue 4, pp. C977-87, (2003) (PubMed).

Hertel, Lacaille, Strobl, Mellins, Mocarski: "Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus." in: Journal of virology, Vol. 77, Issue 13, pp. 7563-74, (2003) (PubMed).

Tachimoto, Kikuchi, Hudson, Bickel, Hamilton, Bochner: "Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases." in: American journal of respiratory cell and molecular biology, Vol. 26, Issue 6, pp. 645-9, (2002) (PubMed).

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).

Nagaraju, Raben, Merritt, Loeffler, Kirk, Plotz: "A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli." in: Clinical and experimental immunology, Vol. 113, Issue 3, pp. 407-14, (1998) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: CD54

Hintergrund: Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

Gen-ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling