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CD43 Antikörper

Dieses Anti-CD43-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD43 in FACS, WB, IHC (p) und IP. Geeignet für Human. Dieses Primary Antibody wurde in 6+ Publikationen zitiert.
Produktnummer ABIN94116

Kurzübersicht für CD43 Antikörper (ABIN94116)

Target

Alle CD43 (SPN) Antikörper anzeigen
CD43 (SPN) (Sialophorin (SPN))

Reaktivität

  • 134
  • 36
  • 18
  • 1
  • 1
Human

Wirt

  • 83
  • 73
  • 27
Maus

Klonalität

  • 113
  • 66
  • 4
Monoklonal

Konjugat

  • 79
  • 17
  • 17
  • 16
  • 10
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD43 Antikörper ist unkonjugiert

Applikation

  • 109
  • 91
  • 59
  • 46
  • 44
  • 23
  • 18
  • 12
  • 6
  • 6
  • 6
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon

MEM-59
  • Verwendungszweck

    Anti-Hu CD43 Purified

    Spezifität

    The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

    Kreuzreaktivität (Details)

    Human

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Human T lymphocytes.

    Isotyp

    IgG1
  • Applikationshinweise

    Flow cytometry: Recommended dilution: 1-5 μg/mL.
    Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil, recommended dilution: 10 μg/mL.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

    Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

    Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

    Alvarado, Klassen, Cerny, Horejsí, Schmidt: "MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation." in: European journal of immunology, Vol. 25, Issue 4, pp. 1051-5, (1995) (PubMed).

    Stefanová, Hilgert, Angelisová, Kristofová, Horejsí: "Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin." in: Folia biologica, Vol. 34, Issue 4, pp. 255-65, (1989) (PubMed).

  • Target

    CD43 (SPN) (Sialophorin (SPN))

    Andere Bezeichnung

    CD43

    Hintergrund

    Sialophorin,CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.,Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5

    Gen-ID

    6693

    UniProt

    P16150

    Pathways

    Regulation of Leukocyte Mediated Immunity
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