CD4 Antikörper (N-Term) (PE)

Produktnummer ABIN94091

Der Maus Monoklonal Anti-CD4-Antikörper wurde für FACS validiert. Er ist geeignet, CD4 in Proben von Human zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für CD4 Antikörper (N-Term) (PE) (ABIN94091)

Target: CD4

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: MEM-241

Produktdetails anti-CD4 Antikörper (PE)

Bindungsspezifität: N-Term

Verwendungszweck: Anti-Hu CD4 PE

Spezifität: The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (""helper"" T-cells) and also on monocytes, tissue macrophages and granulocytes.

Kreuzreaktivität (Details): Human, Other not tested

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD4 Antikörper (PE) (ABIN94091)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Anderson, Sayers, Haniffa, Swan, Diboll, Wang, Isaacs, Hilkens: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, (2008) (PubMed).

Karlsson, Cowley, Martinez, Shaw, Minger, James: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, (2008) (PubMed).

Manasa, Musabaike, Masimirembwa, Burke, Luthy, Mudzori: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, (2007) (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, (2007) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund: CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4

Gen-ID: 920

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events