CD147 Antikörper

Produktnummer ABIN93979

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD147 in FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Schwein und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für CD147 Antikörper (ABIN93979)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD147 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: MEM-M6-2

Produktdetails anti-CD147 Antikörper

Verwendungszweck: Anti-Hu CD147 Purified

Spezifität: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells, it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Kreuzreaktivität (Details): Human, Porcine

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 4 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD147 Antikörper (ABIN93979)

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Details zu CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Andere Bezeichnung: CD147

Hintergrund: Basigin (Ok blood group),CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.,Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group

Gen-ID: 682

UniProt: P35613

Pathways: S100 Proteine