ACRV1 Antikörper

Produktnummer ABIN93928

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ACRV1 in WB und ICC. Er zeigt eine Reaktivität gegenüber Hund und wurde in 1 Publikation erwähnt.

Kurzübersicht für ACRV1 Antikörper (ABIN93928)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reaktivität: Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACRV1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Klon: Ds-2

Produktdetails anti-ACRV1 Antikörper

Verwendungszweck: Anti-Can Intra-Acrosomal Protein Purified

Spezifität: The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.

Kreuzreaktivität (Details): Canine (Dog)

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-ACRV1 Antikörper (ABIN93928)

Geussová, P?knicová, Capková, Kaláb, Moos, Philimonenko, Hozák: "Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction." in: Andrologia, Vol. 29, Issue 5, pp. 261-8, (1997) (PubMed).

Details zu ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Andere Bezeichnung: Intra-Acrosomal Protein

Hintergrund: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,IAP Can