alpha Fetoprotein Antikörper

Produktnummer ABIN93887

Der Maus Monoklonal anti-alpha Fetoprotein Antikörper wird verwendet zum Nachweis von alpha Fetoprotein in Proben von Human. Er wurde validiert für WB, ELISA, ICC, IHC (p) und RIA.

Kurzübersicht für alpha Fetoprotein Antikörper (ABIN93887)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Klon: AFP-11

Produktdetails anti-alpha Fetoprotein Antikörper

Verwendungszweck: Anti-alpha-Fetoprotein Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: purified alpha-Fetoprotein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: hepatocellular carcinoma, heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1 hour / RT, detection DAB.
Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: alpha-Fetoprotein

Hintergrund: Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP

Gen-ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process