PsbO Antikörper

Produktnummer ABIN7676202

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-PsbO Antikörper (ABIN7676202) detektiert spezifisch PsbO in IP, IHC und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Gerste, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum und Mais (Zea mays).

Kurzübersicht für PsbO Antikörper (ABIN7676202)

Target: PsbO

Reaktivität: Arabidopsis thaliana, Gerste, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

Kreuzreaktivität (Details): Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Homologie: Brassica oleracea,Pisum sativum,Populus tremula,Picea sitcHensis,Vitis vinifera

Aufreinigung: Serum

Immunogen: N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321

Anwendungsinformationen

Applikationshinweise: 1 : 1000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).

Antigendetails

Target: PsbO

Hintergrund:

Background: The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Additional information: Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody

Molekulargewicht: 33 kDa

Gen-ID: 836789

UniProt: P23321, Q9S841