KLH Antikörper

Produktnummer ABIN93503

Der Kaninchen Polyklonal Anti-KLH-Antikörper wurde für ELISA, WB und IL validiert. Er ist geeignet, KLH in Proben von Keyhole Limpet zu detektieren.

Kurzübersicht für KLH Antikörper (ABIN93503)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reaktivität: Keyhole Limpet

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-KLH Antikörper

Homologie: Megathura crenulata - most commonly used carrier protein

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: Aprox. 400 kDa

Aufreinigung: serum

Immunogen: purified keyhole limpet hemocyanin (KLH), whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 10 000 (ELISA), 1 : 10 000 (WB), 1 : 1000 (IL).

Kommentare:

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution please add 50 µL of dest. water

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Details zu KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Hintergrund: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Molekulargewicht: ca. 400 kDa/subunit

UniProt: Q6KC56