KLH Antikörper

Produktnummer ABIN93503

Der Kaninchen Polyklonal anti-KLH Antikörper wird verwendet zum Nachweis von KLH in Proben von Keyhole Limpet. Er wurde validiert für ELISA, WB und IL.

Kurzübersicht für KLH Antikörper (ABIN93503)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reaktivität: Keyhole Limpet

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLH Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-KLH Antikörper

Verwendungszweck: Anti-KLH | Keyhole limpet hemocyanin

Kreuzreaktivität (Details): Megathura crenulata - most commonly used carrier protein
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Aufreinigung: Immunogen affinity purified serum

Immunogen: Purified keyhole limpet hemocyanin (KLH)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

Kommentare:

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Hintergrund:

Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Additional information: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

Molekulargewicht: ca. 400 kDa/subunit

UniProt: Q6KC56