T7-Tag Antikörper (HRP)

Produktnummer ABIN920811

Der HRP-konjugierte Kaninchen Polyklonal anti-T7-Tag Antikörper (ABIN920811) detektiert spezifisch T7-Tag in WB und ELISA.

Kurzübersicht für T7-Tag Antikörper (HRP) (ABIN920811)

Target: T7-Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser T7-Tag Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-T7-Tag Antikörper (HRP)

Sequenz: MASMTGGQQM G

Anwendungsinformationen

Applikationshinweise: Western blot: Use at 1:5,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 mM HEPES pH 7.0, 0.1 M sodium chloride with 0.2% BSA and 0.01% thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu T7-Tag

Target: T7-Tag

Andere Bezeichnung: T7 tag

Substanzklasse: Tag

Hintergrund: Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support and then conjugated with horseradish peroxidase (HRP). Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. ELISA: The anti-T7 diluted 1:25,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier.