T7-Tag Antikörper

Produktnummer ABIN920809

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch T7-Tag in WB und ELISA.

Kurzübersicht für T7-Tag Antikörper (ABIN920809)

Target: T7-Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser T7-Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-T7-Tag Antikörper

Sequenz: MASMTGGQQM G

Aufreinigung: Affinity purified

Anwendungsinformationen

Applikationshinweise: Western blot: Use at 1:5,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.2 and 0.1% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu T7-Tag

Target: T7-Tag

Andere Bezeichnung: T7 tag

Substanzklasse: Tag

Hintergrund: Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.