E Tag Antikörper

Produktnummer ABIN920773

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch E Tag in WB und ELISA.

Kurzübersicht für E Tag Antikörper (ABIN920773)

Target: E Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-E Tag Antikörper

Sequenz: GAPVPYPDPL EPR

Aufreinigung: Affinity purified

Anwendungsinformationen

Applikationshinweise: Western blot: Recommend dilution range 1:1,000 - 1:3,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS pH 7.2 with 0.1% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu E Tag

Target: E Tag

Andere Bezeichnung: E-tag

Substanzklasse: Tag

Hintergrund: Rabbits were immunized with E-tag peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated donkey anti-rabbit (IgG) at 1:18,000 and TMB as the substrate. Appropriate specificity controls were run.