Vimentin Antikörper
Kurzübersicht für Vimentin Antikörper (ABIN920739)
Target
Alle Vimentin (VIM) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells, and has a molecular weight of 57 kDa. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57 kDa vimentin band in immunoblots performed on a lysate of normal human lymphocytes. No detectable cross-reactivity with GFAP, Keratin, Neurofilament or Desmin.
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Aufreinigung
- Purified
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Isotyp
- IgG1
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Applikationshinweise
- Immunohistochemistry: For use on frozen sections and formalin-fixed/paraffin- embedded tissues if pre-treated with trypsin. Paraffin sections: Use at an approximate dilution of 1:50 - 1:100 in PBS. Trypsin digestion may be required. Frozen sections: Use at an approximate dilution of 1:300 in PBS.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- 1 mL of purified monoclonal antibody at approximately 30 µg/mL in PBS containing 20 mM sodium azide, and protein stabilizer.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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- Vimentin (VIM)
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Andere Bezeichnung
- Vimentin
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Pathways
- Caspase Kaskade in der Apoptose
Target
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