Nck1/Nck alpha (AA 31-150) Antikörper (AbBy Fluor® 488)

Produktnummer ABIN907752

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in IF (cc) und IF (p). Geeignet für Human.

Kurzübersicht für Nck1/Nck alpha (AA 31-150) Antikörper (AbBy Fluor® 488) (ABIN907752)

Target: Nck1/Nck alpha

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: AbBy Fluor® 488

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails

Bindungsspezifität: AA 31-150

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nck2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Nck1/Nck alpha

Hintergrund:

Synonyms: NCK adaptor protein 2, Cytoplasmic protein NCK1, Melanoma Nck protein, MGC12668, NCK 1, Nck adaptor protein 1, NCK tyrosine kinase, NCK1, NCKalpha, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK alpha.

Background: Nck is a highly conserved, oncogenic protein. It is a common target for the action of different surface receptors, encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase activating protein, phosphatidylinositol 3 kinase, and phospholipase Cg. Nck is widely expressed in various tissues and in cell lines from human, murine, and rat origins. Nck is phosphorylated on tyrosine, serine, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively. Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells.

Gen-ID: 4690