CD4 Antikörper (AA 385-457) (AbBy Fluor® 555)

Produktnummer ABIN889094

Der Kaninchen Polyklonal Anti-CD4-Antikörper wurde für FACS, WB, IF (cc) und IF (p) validiert. Er ist geeignet, CD4 in Proben von Human, Maus, Ratte, Huhn und Schwein zu detektieren.

Kurzübersicht für CD4 Antikörper (AA 385-457) (AbBy Fluor® 555) (ABIN889094)

Target: CD4

Reaktivität: Human, Maus, Ratte, Huhn, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit AbBy Fluor® 555

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-CD4 Antikörper (AbBy Fluor® 555)

Bindungsspezifität: AA 385-457

Kreuzreaktivität: Huhn, Human, Maus, Schwein, Ratte

Homologie: Dog,Cow,Sheep,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4

Hintergrund:

Synonyms: T-cell surface glycoprotein CD4, Cd4, T-cell surface antigen T4/Leu-3, W3/25 antigen

Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Gen-ID: 24932

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events