Small Basement Membrane Heparan Sulfate Proteoglycan Antikörper

Produktnummer ABIN870825

Der Maus Monoklonal anti-Small Basement Membrane Heparan Sulfate Proteoglycan Antikörper wird verwendet zum Nachweis von Small Basement Membrane Heparan Sulfate Proteoglycan in Proben von Human. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für Small Basement Membrane Heparan Sulfate Proteoglycan Antikörper (ABIN870825)

Target: Small Basement Membrane Heparan Sulfate Proteoglycan

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: 1F10-B8

Produktdetails

Spezifität: Monoclonal Ab 1F10/B8 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). MAb 1F10/B8 recognizes an epitope different from that recognized by mAb 2H7/G11 and mAb 2E2/B5. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

Aufreinigung: Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml). This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Resuspend in aqua bidest.

Buffer: 50 mM TRIS pH 7.4

Lagerung: 4 °C

Antigendetails

Target: Small Basement Membrane Heparan Sulfate Proteoglycan