Small Basement Membrane Heparan Sulfate Proteoglycan Antikörper

Produktnummer ABIN870825

Produktdetails

Target: Small Basement Membrane Heparan Sulfate Proteoglycan

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Spezifität: Monoclonal Ab 1F10/B8 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). MAb 1F10/B8 recognizes an epitope different from that recognized by mAb 2H7/G11 and mAb 2E2/B5. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

Aufreinigung: Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.

Klon: 1F10-B8

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml). This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Resuspend in aqua bidest.

Buffer: 50 mM TRIS pH 7.4

Lagerung: 4 °C

Antigendetails

Target: Small Basement Membrane Heparan Sulfate Proteoglycan