APP Antikörper (N-Term)

Produktnummer ABIN870659

Der Ziege Polyklonal Anti-APP-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, APP in Proben von Human und Maus zu detektieren.

Kurzübersicht für APP Antikörper (N-Term) (ABIN870659)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-APP Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Amyloid beta peptide (mouse)

Sequenz: DAEFGHDSGF EVRHQK

Spezifität: This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DAEFGHDSGFEVRHQK-C, from the N Terminus of the protein sequence according to NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in Mouse Brain lysates (calculated MW of 86.7 kDa according to NP_001185752.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: App

Hintergrund: App, amyloid beta (A4) precursor protein, Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp, alzheimer disease amyloid A4 protein homolog, amyloid beta A4 protein, amyloidogenic glycoprotein, appican, protease nexin II

Molekulargewicht: 86.7kDa

Gen-ID: 11820, 54226

NCBI Accession: NP_001185752, NP_031497, NP_001185753, NP_001185754, NP_001185755

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour