Amyloid Antikörper

Produktnummer ABIN863210

Der Kaninchen Polyklonal Anti--Antikörper wurde für ELISA, IP, IHC, WB, ICC und IF validiert. Er ist geeignet, in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Amyloid Antikörper (ABIN863210)

Target: Amyloid

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails

Spezifität: Recognizes all types of amyloid oligomers. Appears to recog a peptide backbone epitope common to amyloid oligomers, but not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.

Kreuzreaktivität: Eukaryonten, Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Synthetic molecular mimic of soluble oligomers

Anwendungsinformationen

Applikationshinweise:

• WB (1:200)
• IP (1:200)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:200 dilution of ABIN863210 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen

Voth, Gwin, Francis, Balczon, Frank, Pittet, Wagener, Moser, Alexeyev, Housley, Audia, Piechocki, Madera, Simmons, Crawford, Stevens: "Virulent Pseudomonas aeruginosa infection converts antimicrobial amyloids into cytotoxic prions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 34, Issue 7, pp. 9156-9179, (2020) (PubMed).

Epelbaum, Youssef, Lacor, Chaurand, Duplus, Brugg, Duyckaerts, Delatour: "Acute amnestic encephalopathy in amyloid-β oligomer-injected mice is due to their widespread diffusion in vivo." in: Neurobiology of aging, Vol. 36, Issue 6, pp. 2043-52, (2015) (PubMed).

Antigendetails

Target: Amyloid

Hintergrund: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).

Gen-ID: 8666

NCBI Accession: NM_000484

UniProt: P05067