SLC9A3 Antikörper

Produktnummer ABIN863199

Dieses Anti-SLC9A3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SLC9A3 in WB, IHC, ICC und IF. Geeignet für Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für SLC9A3 Antikörper (ABIN863199)

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC9A3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-SLC9A3 Antikörper

Spezifität: Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN863199 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-SLC9A3 Antikörper (ABIN863199)

Jones, Bailey, Murray, Lu, McNeilly, Schlötzer-Schrehardt, Lennon, Sado, Brownstein, Mullins, Kadler, Van Agtmael: "ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice." in: Disease models & mechanisms, Vol. 9, Issue 2, pp. 165-76, (2016) (PubMed).

Lee, Osis, Handlogten, Guo, Verlander, Weiner: "Effect of dietary protein restriction on renal ammonia metabolism." in: American journal of physiology. Renal physiology, Vol. 308, Issue 12, pp. F1463-73, (2015) (PubMed).

Bourgeois, Bounoure, Christensen, Ramakrishnan, Houillier, Devuyst, Wagner: "Haploinsufficiency of the ammonia transporter Rhcg predisposes to chronic acidosis: Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct." in: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5518-29, (2013) (PubMed).

Details zu SLC9A3

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Andere Bezeichnung: NHE3

Hintergrund: Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).

Gen-ID: 24784

NCBI Accession: NP_036786

UniProt: P26433

Pathways: Proton Transport