SHANK3 Antikörper (AA 840-857)

Produktnummer ABIN863129

Der Maus Monoklonal Anti-SHANK3-Antikörper wurde für WB, IHC, IP, ICC, IF und AA validiert. Er ist geeignet, SHANK3 in Proben von Ratte zu detektieren.

Kurzübersicht für SHANK3 Antikörper (AA 840-857) (ABIN863129)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SHANK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Klon: S69

Produktdetails anti-SHANK3 Antikörper

Bindungsspezifität: AA 840-857

Spezifität: Detects ~190 kDa. No cross-reactivity against Shank1 or Shank2.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide amino acids 840-857 of rat Shank3

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN863129 was sufficient for detection of Shank3 in 10 μg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Andere Bezeichnung: SHANK3

Hintergrund: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling, specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).

Gen-ID: 59312

NCBI Accession: NP_067708

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity