P-Selectin Antikörper (FITC)

Produktnummer ABIN810080

Produktdetails anti-P-Selectin Antikörper (FITC)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Selectin Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Verwendungszweck: Anti-Hu CD62P FITC

Spezifität: The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes, it is relocated to the plasma membrane upon activation.

Kreuzreaktivität (Details): Human, Non-Human Primates

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human platelets

Klon: AK4

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-P-Selectin Antikörper (FITC) (ABIN810080)

Ludwig, Schultz, Boehncke, Podda, Tandi, Krombach, Baatz, Kaufmann, von Andrian, Zollner: "Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules." in: The Journal of investigative dermatology, Vol. 122, Issue 3, pp. 830-6, (2004) (PubMed).

Kowalska, Ratajczak, Hoxie, Brass, Gewirtz, Poncz, Ratajczak: "Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets." in: British journal of haematology, Vol. 104, Issue 2, pp. 220-9, (1999) (PubMed).

Holme, Müller, Solum, Brosstad, Frøland, Aukrust: "Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 12, Issue 1, pp. 79-89, (1998) (PubMed).

Dunlop, Skinner, Bendall, Favaloro, Castaldi, Gorman, Gamble, Vadas, Berndt: "Characterization of GMP-140 (P-selectin) as a circulating plasma protein." in: The Journal of experimental medicine, Vol. 175, Issue 4, pp. 1147-50, (1992) (PubMed).

Details zu P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Andere Bezeichnung: CD62P (SELP Produkte)

Hintergrund: Selectin P,CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.,P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL

Gen-ID: 6403

UniProt: P16109