LY75/DEC-205 Antikörper (PE)

Produktnummer ABIN807925

Dieses Maus Monoklonal-Antikörper erkennt spezifisch LY75/DEC-205 in FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für LY75/DEC-205 Antikörper (PE) (ABIN807925)

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LY75/DEC-205 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: HD30

Produktdetails anti-LY75/DEC-205 Antikörper (PE)

Verwendungszweck: Anti-Hu CD205 PE

Spezifität: The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant Fc-tagged human CD205

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-LY75/DEC-205 Antikörper (PE) (ABIN807925)

Park, Rodriguez, Ueta, Lee, Pack, Matsuno, Steinman: "Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals." in: Journal of immunological methods, Vol. 377, Issue 1-2, pp. 15-22, (2012) (PubMed).

Details zu LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Andere Bezeichnung: CD205

Hintergrund: Lymphocyte antigen 75,CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.,DEC-205, LY75, CLEC13B

Gen-ID: 4065

UniProt: O60449