CXCR5 Antikörper

Produktnummer ABIN796835

Der Ratte Monoklonal anti-CXCR5 Antikörper wird verwendet zum Nachweis von CXCR5 in Proben von Maus. Er wurde validiert für FACS, ELISA und ICC.

Kurzübersicht für CXCR5 Antikörper (ABIN796835)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CXCR5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC)

Klon: 614641

Produktdetails anti-CXCR5 Antikörper

Spezifität: Mouse CXCR5.

Aufreinigung: Protein G purified

Immunogen: Recombinant corresponding to mouse CXCR5 expressed in E. coli (Q04683)

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, ICC

Usage: Flow Cytometry: 2.5 μg per 10e6 cells, Mouse splenocytes werestained with catalog #LS-C143773 followed by PerCP-conjugatedanti-Rat IgG F(ab')2 secondary antibody and a mouseB220/CD45R PE-conjugated monoclonal antibody.Immunocytochemistry: 8-25 μg/mL, CXCR5 was detected inimmersion fixed A20 mouse B cell lymphoma cell line usingcatalog #LS-C143773 at 10 μg/mL for 3 hours at roomtemperature. Cells were stained using the fluorescent-conjugatedanti-Rat IgG secondary antibody and counterstained with DAPI.Staining was localized to cell surfaces and cytoplasm.Optimal dilutions to be determined by the researcher.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Details zu CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Andere Bezeichnung: CXCR5

Hintergrund: Name/Gene ID: CXCR5
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR5, CD185 antigen, CD185, CXCR-5, CXC-R5, GPR9, MDR15, Monocyte-derived receptor 15, Blr-1, BLR1, Burkitt lymphoma receptor 1, Burkitt's lymphoma receptor 1, MDR-15

Gen-ID: 643

UniProt: P32302