Streptavidin Antikörper

Produktnummer ABIN398439

Dieses Anti-Streptavidin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Streptavidin in ELISA und WB. Geeignet für Streptomyces avidinii. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Streptavidin Antikörper (ABIN398439)

Target: Streptavidin

Reaktivität: Streptomyces avidinii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Streptavidin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-Streptavidin Antikörper

Spezifität: Streptavidin Antibody is specific to streptavidin.

Aufreinigung: Protein G chromatography

Immunogen: Purified recombinant streptavidin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.5-1 µg/mL Dot blot: 0.5-1 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Streptavidin Antikörper (ABIN398439)

Snyder, Kellogg, Skarda, Little, Kristich: "Nutritional control of antibiotic resistance via an interface between the phosphotransferase system and a two-component signaling system." in: Antimicrobial agents and chemotherapy, Vol. 58, Issue 2, pp. 957-65, (2014) (PubMed).

Lorey, Fiedler, Kunert, Nerkamp, Lange, Fiedler, Bosse-Doenecke, Meysing, Gloser, Rundfeldt, Rauchhaus, Hänssgen, Göttler, Steuernagel, Fiedler, Haupts: "Novel ubiquitin-derived high affinity binding proteins with tumor targeting properties." in: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8493-507, (2014) (PubMed).

Yonekita, Ohtsuki, Hojo, Morishita, Matsumoto, Aizawa, Morimatsu: "Development of a novel multiplex lateral flow assay using an antimicrobial peptide for the detection of Shiga toxin-producing Escherichia coli." in: Journal of microbiological methods, Vol. 93, Issue 3, pp. 251-6, (2013) (PubMed).

Details zu Streptavidin

Target: Streptavidin

Hintergrund: Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of almost equal to10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin Antibody is developed in rabbit using purified Streptavidin and highly purified from rabbit antiserum by protein G resin.