AQP2 Antikörper (pSer269)

Produktnummer ABIN784597

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AQP2 in WB und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Ratte, Maus, Hund und Rind (Kuh).Es wurde in 1 Publikation zitiert.

Kurzübersicht für AQP2 Antikörper (pSer269) (ABIN784597)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte, Maus, Hund, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: pSer269

Spezifität: Specific for ~29k AQP2 protein phosphorylated at Ser269. This antibody also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Mouse.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of Rat Aquaporin 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Referenzen für anti-AQP2 Antikörper (ABIN784597)

Bogum, Faust, Zühlke, Eichhorst, Moutty, Furkert, Eldahshan, Neuenschwander, von Kries, Wiesner, Trimpert, Deen, Valenti, Rosenthal, Klussmann: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aquaporin-2

Hintergrund: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, Serine-269 has been identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Gen-ID: 25386

NCBI Accession: NP_037041

Pathways: Response to Water Deprivation