AQP2 Antikörper (pSer269)

Produktnummer ABIN784597

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AQP2 in WB und IHC (fro). Er zeigt eine Reaktivität gegenüber Ratte, Maus, Rind (Kuh) und Hund und wurde in 1 Publikation erwähnt.

Kurzübersicht für AQP2 Antikörper (pSer269) (ABIN784597)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Ratte, Maus, Rind (Kuh), Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: pSer269

Spezifität: Specific for ~29k AQP2 protein phosphorylated at Ser269. This antibody also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Mouse.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of Rat Aquaporin 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Referenzen für anti-AQP2 Antikörper (ABIN784597)

Bogum, Faust, Zühlke, Eichhorst, Moutty, Furkert, Eldahshan, Neuenschwander, von Kries, Wiesner, Trimpert, Deen, Valenti, Rosenthal, Klussmann: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aquaporin-2

Hintergrund: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, Serine-269 has been identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Gen-ID: 25386

NCBI Accession: NP_037041

Pathways: Response to Water Deprivation