AQP2 Antikörper (pSer264)

Produktnummer ABIN784596

Dieses Anti-AQP2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von AQP2 in WB und IHC (fro). Geeignet für Human, Ratte, Maus, Rind (Kuh), Hund, Huhn und Non-Human Primate. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für AQP2 Antikörper (pSer264) (ABIN784596)

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund, Huhn, Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AQP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AQP2 Antikörper

Bindungsspezifität: pSer264

Spezifität: Specific for ~29k AQP2 protein phosphorylated at Ser264. This antibody also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Aufreinigung: Affinity Chromatography.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser264 of Rat Aquaporin 2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Referenzen für anti-AQP2 Antikörper (ABIN784596)

Hoffert, Fenton, Moeller, Simons, Tchapyjnikov, McDill, Yu, Pisitkun, Chen, Knepper: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).

Details zu AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Andere Bezeichnung: Aquaporin-2

Hintergrund: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-264 has been identified as a novel phosphorylation site on AQP2 and shown to be regulated by vasopressin thus implicating this site as important in AQP2 trafficking and subcellular distribution (Fenton RA et al., 2008).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Gen-ID: 25386

NCBI Accession: NP_037041

Pathways: Response to Water Deprivation