Tryptophan Hydroxylase 2 Antikörper (N-Term)

Produktnummer ABIN784279

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Tryptophan Hydroxylase 2 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund, Rind (Kuh) und Schwein.

Kurzübersicht für Tryptophan Hydroxylase 2 Antikörper (N-Term) (ABIN784279)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Tryptophan Hydroxylase 2 Antikörper

Bindungsspezifität: AA 16-29, N-Term

Sequenz: RGFSLDSAVP EEHQ-C

Spezifität: Recognizes TPH2 (16-29).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_775489.2. Genename: TPH2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Andere Bezeichnung: Tryptophan 5-Hydroxylase 2 (TPH2)

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

Gen-ID: 121278

NCBI Accession: NP_775489