Der Kaninchen Polyklonal anti-Transferrin Antikörper wird verwendet zum Nachweis von Transferrin in Proben von Schwein. Er wurde validiert für WB, IP, IF, EIA, ID, IHC (fro) und DB.
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE-chromatography.
Immunogen
Purified Transferrin isolated from pooled Swine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water
Konzentration
10 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. Avoid Repeated thawing and freezing.
Target
Transferrin (TF)
Hintergrund
Transferrin is a single polypeptide chain glycoprotein belonging to iron binding family of proteins. It has a molecular weight of 81,5 kDa (porcine). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin