RNF8 Antikörper (C-Term)

Produktnummer ABIN783604

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RNF8 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für RNF8 Antikörper (C-Term) (ABIN783604)

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RNF8 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: 14 amino acid peptide near the carboxy terminus of human RNF8

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Andere Bezeichnung: RNF8

Hintergrund: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.Synonyms: E3 ubiquitin-protein ligase RNF8, RING finger protein 8

Gen-ID: 9025

NCBI Accession: NP_003949

UniProt: O76064

Pathways: Production of Molecular Mediator of Immune Response