GEMC1 Antikörper (Center)

Produktnummer ABIN783498

Der Kaninchen Polyklonal anti-GEMC1 Antikörper (ABIN783498) detektiert spezifisch GEMC1 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für GEMC1 Antikörper (Center) (ABIN783498)

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GEMC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-GEMC1 Antikörper

Bindungsspezifität: Center

Spezifität: This antibody detects GEMC1 at Center.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the center of human GEMC1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azi

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Details zu GEMC1

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Andere Bezeichnung: GEMC1

Hintergrund: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.Synonyms: Geminin coiled-coil domain-containing protein 1

Gen-ID: 647309

NCBI Accession: NP_001140158

UniProt: A6NCL1