Tryptophan Hydroxylase 2 Antikörper (Internal Region)

Produktnummer ABIN768606

Dieses Anti-Tryptophan Hydroxylase 2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Tryptophan Hydroxylase 2 in ELISA und IF. Geeignet für Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Tryptophan Hydroxylase 2 Antikörper (Internal Region) (ABIN768606)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Tryptophan Hydroxylase 2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Tph2 (mouse)

Sequenz: SLTQNKAIKS EDK

Kreuzreaktivität: Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SLTQNKAIKSEDK, from the internal region of the protein sequence according to NP_775567.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave an approx 85 kDa band in Mouse fetal Brain lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: In free-floating samples of Mouse Brain stem staining of Raphe nucleii serotonergic neurons can be seen, that is consistent with observation by different antibodies. Recommended concentration, 0.5-2μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Tryptophan Hydroxylase 2 Antikörper (ABIN768606)

Rahim, Meedeniya, Crane: "Central serotonergic neuron deficiency in a mouse model of Zellweger syndrome." in: Neuroscience, Vol. 274, pp. 229-41, (2014) (PubMed).

Details zu Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Andere Bezeichnung: Tph2

Hintergrund: Tph2, tryptophan hydroxylase 2, AU043594, MGC159133, Ntph, OTTMUSP00000022885, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2

Molekulargewicht: 85kDa

Gen-ID: 216343

NCBI Accession: NP_775567