PROL1 Antikörper (Internal Region)

Produktnummer ABIN768578

Der Ziege Polyklonal Anti-PROL1-Antikörper wurde für WB, IHC, IP, ELISA und IF validiert. Er ist geeignet, PROL1 in Proben von Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für PROL1 Antikörper (Internal Region) (ABIN768578)

Target: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PROL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-PROL1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Mucin 10 / Prol1

Sequenz: QFPVRKYLED PRY

Kreuzreaktivität: Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QFPVRKYLEDPRY, from the internal region of the protein sequence according to NP_032670.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publi

Western Blot: This antibody has been successfully used in WB on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: This antibody has been successfully used in IF on Mouse: Peluso G et al. (2019) J Biol Chem. 2019 Dec 27. pii: jbc.RA119.009807. PMID: 31882545.

Immunoprecipitation: This antibody has been successfu

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PROL1 Antikörper (ABIN768578)

Tanaka, Ogawa, Hojo, Kawashima, Mabuchi, Hata, Nakamura, Yasuhara, Takamatsu, Irié, Fukada, Sakai, Inoue, Nishimura, Ohara, Saito, Ohba, Tsuji, Mishima: "Generation of orthotopically functional salivary gland from embryonic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 4216, (2019) (PubMed).

Nelson, Manhardt, Kamath, Sui, Santamaria-Pang, Can, Bello, Corwin, Dinn, Lazare, Gervais, Sequeira, Peters, Ginty, Gerdes, Larsen: "Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation." in: Biology open, Vol. 2, Issue 5, pp. 439-47, (2013) (PubMed).

Details zu PROL1

Target: PROL1 (Proline Rich, Lacrimal 1 (PROL1))

Andere Bezeichnung: Prol1

Hintergrund: Prol1, proline rich, lacrimal 1, Muc10, mucin 10, mucin 10, submandibular gland salivary mucin

Molekulargewicht: 45kDa

Gen-ID: 17830

NCBI Accession: NP_032670