SIRT3 Antikörper (Internal Region)

Produktnummer ABIN768568

Dieser Ziege Polyklonal Antikörper erkennt spezifisch SIRT3 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für SIRT3 Antikörper (Internal Region) (ABIN768568)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SIRT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-SIRT3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Sirtuin 3 (mouse)

Sequenz: RASGIPASKL VEAH

Spezifität: This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1

Kreuzreaktivität: Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2, NP_001171275.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 30 kDa band observed in Mouse Heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Andere Bezeichnung: Sirt3

Hintergrund: Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3

Molekulargewicht: 28.8kDa according to NP_071878.2

Gen-ID: 23410, 64384, 293615

NCBI Accession: NP_071878, NP_001171275