DNAJB9 Antikörper (AA 61-75)

Produktnummer ABIN768557

Der Ziege Polyklonal Anti-DNAJB9-Antikörper wurde für WB, ELISA, IF und FACS validiert. Er ist geeignet, DNAJB9 in Proben von Human und Maus zu detektieren.

Kurzübersicht für DNAJB9 Antikörper (AA 61-75) (ABIN768557)

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DNAJB9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-DNAJB9 Antikörper

Bindungsspezifität: AA 61-75

Verwendungszweck: DNAJB9 (aa61-75)

Sequenz: PDAEAKFREI AEAYE

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PDAEAKFREIAEAYE, from the internal regoin of the protein sequence according to NP_036460.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx.28 kDa in Mouse Liver lysates and in preliminary testing of Rat Liver lysate (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DNAJB9

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Andere Bezeichnung: DNAJB9

Hintergrund: DNAJB9, DnaJ (Hsp40) homolog, subfamily B, member 9, DKFZp564F1862, ERdj4, MDG1, MST049, MSTP049, dnaJ homolog subfamily B member 9, endoplasmic reticulum DnaJ homolog 4, mdg-1, microvascular endothelial differentiation gene 1 protein

Molekulargewicht: 25.5kDa according to NP_036460.1

Gen-ID: 4189

NCBI Accession: NP_036460

Pathways: ER-Nucleus Signaling