OCT4 Antikörper

Produktnummer ABIN8123909

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch OCT4 in WB, ELISA, IHC, IF und IP. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für OCT4 Antikörper (ABIN8123909)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AB1932

Produktdetails anti-OCT4 Antikörper

Verwendungszweck: Oct-4 Rabbit mAb

Spezifität: This antibody detects endogenous levels of OCT-4 protein.

Aufreinigung: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB, 1:2000-1:10000 | IHC, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at-20°C. Avoid freeze / thaw cycles.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: Oct-4

Hintergrund:

Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3

Background: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing&apos, s sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013],

Gene Name: POU5F1

Protein Name: OCT-4

Gen-ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance