MMP1 Antikörper (Cleaved-Ile271)

Produktnummer ABIN8122794

Der Kaninchen Polyklonal anti-MMP1 Antikörper wird verwendet zum Nachweis von MMP1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für MMP1 Antikörper (Cleaved-Ile271) (ABIN8122794)

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-MMP1 Antikörper

Bindungsspezifität: Cleaved-Ile271

Verwendungszweck: MMP1 (Cleaved-Ile271) Antibody

Spezifität: This antibody detects endogenous levels of Human MMP1 (Cleaved-Ile271, protein was cleaved amino acid sequence between 270-271 )

Aufreinigung: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human MMP1 (Cleaved-Ile271)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-2000,IHC 1:50-300, ELISA 2000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu MMP1

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: MMP1

Hintergrund:

Synonyms: Interstitial collagenase (EC 3.4.24.7, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]

Background: catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,domain:There are two distinct domains in this protein, the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).,enzyme regulation:Can be activated without removal of the activation peptide.,function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.,online information:Collagenase entry,PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subunit:Interacts with HIV-1 Tat.,

Gene Name: MMP1 CLG

Protein Name: MMP1 (Cleaved-Ile271)

Gen-ID: 4312

UniProt: P03956