GPX4 Antikörper

Produktnummer ABIN8121712

Der Kaninchen Monoklonal anti-GPX4 Antikörper (Klon AB2084) (ABIN8121712) detektiert spezifisch GPX4 in WB, ELISA, IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für GPX4 Antikörper (ABIN8121712)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser GPX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AB2084

Produktdetails anti-GPX4 Antikörper

Verwendungszweck: GPX4 Rabbit mAb

Spezifität: This antibody detects endogenous levels of GPX4 protein.

Aufreinigung: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at-20°C. Avoid freeze / thaw cycles.

Details zu GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Andere Bezeichnung: GPX4

Hintergrund:

Synonyms: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4,

Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development, thus, it has been identified as a &apos, moonlighting&apos, protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS) . This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3&apos, UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016],

Gene Name: GPX4

Protein Name: GPX4

Gen-ID: 2879

UniProt: P36969