AF4 Antikörper (AA 31-130)

Produktnummer ABIN760376

Der Kaninchen Polyklonal Anti-AF4-Antikörper wurde für ELISA, IF (cc), IF (p), IHC (p) und IHC (fro) validiert. Er ist geeignet, AF4 in Proben von Human zu detektieren.

Kurzübersicht für AF4 Antikörper (AA 31-130) (ABIN760376)

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AF4 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-AF4 Antikörper

Bindungsspezifität: AA 31-130

Homologie: Human,Mouse,Rat,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AF 4 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu AF4

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Andere Bezeichnung: Af 4 Protein

Hintergrund:

Synonyms: AF 4, AF 4 protein, AF-4 protein, AF4/FMR2 family member 1, AFF 1, AFF1, AFF1_HUMAN, ALL1 fused gene from chromosome 4, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2, MYELOID/LYMPHOID OR MIXED LINEAGE LEUKEMIA TRANSLOCATED TO 2, MLLT2, Protein AF-4, Protein FEL, Proto-oncogene AF4.

Background: Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells. The expression of MLL-AF-4 influences the production of protein cyclin-dependent kinase inhibitor (CDKN1B), suggesting that inhibition of MLL-AF-4 expression may be a powerful and highly specific treatment of chemotherapy-resistant leukemia.

Gen-ID: 4299