SRP72 Antikörper (AA 40-460)

Produktnummer ABIN8093474

Der Kaninchen Polyklonal anti-SRP72 Antikörper wird verwendet zum Nachweis von SRP72 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, FACS, ICC, IF, IHC (p) und IP.

Kurzübersicht für SRP72 Antikörper (AA 40-460) (ABIN8093474)

Target: SRP72 (Signal Recognition Particle 72kDa (SRP72))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRP72 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-SRP72 Antikörper

Bindungsspezifität: AA 40-460

Verwendungszweck: SRP72 Antibody / Signal recognition particle 72 kDa protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human SRP72 recombinant protein (amino acids I40-Y460) was used as the immunogen for the SRP72 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SRP72 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SRP72 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SRP72

Target: SRP72 (Signal Recognition Particle 72kDa (SRP72))

Andere Bezeichnung: SRP72

Hintergrund: SRP72 antibody recognizes Signal recognition particle 72 kDa protein, a core component of the signal recognition particle (SRP) complex responsible for co-translational targeting of secretory and membrane proteins. Encoded by the SRP72 gene on chromosome 4q12, this protein forms part of the SRP heterodimer with SRP68 and contributes to binding ribosome-nascent chain complexes. SRP72 plays a central role in directing nascent polypeptides to the endoplasmic reticulum (ER) membrane, ensuring proper insertion, folding, and trafficking of secreted and transmembrane proteins. It is expressed in most tissues, with high levels found in cells with active secretory or membrane protein synthesis. SRP72 localizes primarily to the cytoplasm where it co-localizes with ribosomes, SRP RNA, and translational components.

Signal recognition particle 72 kDa protein participates in a conserved pathway involving recognition of N-terminal signal peptides emerging from the ribosome. SRP72 stabilizes the SRP complex and contributes to structural rearrangements required for pausing translation and initiating ER targeting. Binding of SRP to the signal peptide-ribosome complex enables recruitment of the SRP receptor at the ER membrane, followed by transfer of the nascent chain to the Sec61 translocon. Through these steps, SRP72 supports accurate protein sorting, prevents mislocalization of secretory proteins, and maintains overall proteostasis within the secretory pathway.

Dysfunction in SRP pathway components, including SRP72, can lead to defects in protein trafficking and ER stress. Mutations in SRP72 have been associated with a rare form of familial aplasia and myelodysplasia, where impaired SRP function disrupts hematopoietic stem cell viability and differentiation. Experimental studies suggest that SRP72 mutations alter ribosome-binding or structural integrity of the SRP complex, contributing to defective protein targeting. Altered SRP72 expression has also been linked to cancer biology, with evidence implicating the SRP machinery in supporting rapid protein synthesis and secretory demands of tumor cells. Additionally, SRP72 participates in stress-related translational responses, where modulation of SRP activity influences ER homeostasis and adaptive signaling pathways.

At the subcellular level, SRP72 localizes to the cytoplasm and associates with ribosome-rich regions, ER membranes, and protein synthesis hubs. It co-localizes with SRP68, SRP54, and 7SL RNA, forming a functional SRP complex essential for co-translational targeting. Structural studies show that SRP72 contributes to the architecture required for ribosome interaction and SRP receptor engagement. Isoform variation resulting from alternative splicing may influence nucleo-cytoplasmic transport, RNA binding affinity, or interactions with other SRP components. Developmentally, SRP72 expression increases as secretory pathways mature and supports expanding protein synthesis demands in differentiating tissues.

This SRP72 antibody is suitable for detecting Signal recognition particle 72 kDa protein in research focused on protein trafficking, ER targeting, translation regulation, hematopoietic disorders, and secretory pathway biology. It supports studies examining SRP complex function, co-translational sorting, and proteostasis under stress or disease conditions. NSJ Bioreagents provides this antibody as part of its protein synthesis and cell biology collection.

UniProt: O76094

Pathways: SARS-CoV-2 Protein Interaktom