SRP54 Antikörper (AA 2-433)

Produktnummer ABIN8093473

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SRP54 in WB, FACS, IP und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für SRP54 Antikörper (AA 2-433) (ABIN8093473)

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SRP54 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-SRP54 Antikörper

Bindungsspezifität: AA 2-433

Verwendungszweck: SRP54 Antibody / Signal recognition particle 54 kDa protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human Signal recognition particle 54 kDa protein (amino acids V2-K433) was used as the immunogen for the SRP54 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the SRP54 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the SRP54 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu SRP54

Target: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Andere Bezeichnung: SRP54

Hintergrund: SRP54 antibody targets Signal recognition particle 54 kDa protein, encoded by the SRP54 gene. Signal recognition particle 54 kDa protein is a core component of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates co-translational targeting of nascent polypeptides to the endoplasmic reticulum membrane. SRP54 is primarily localized in the cytoplasm and associates transiently with ribosomes, where it recognizes signal peptides emerging from the ribosomal exit tunnel. This interaction is essential for directing secretory and membrane proteins into the correct cellular trafficking pathway.

Functionally, Signal recognition particle 54 kDa protein serves as the signal peptide-binding and GTPase subunit of the SRP complex. Upon binding a signal sequence, SRP54 pauses translation and facilitates docking of the ribosome-nascent chain complex to the SRP receptor at the endoplasmic reticulum membrane. Subsequent GTP-dependent interactions between SRP54 and the SRP receptor coordinate transfer of the translating ribosome to the translocon, allowing protein synthesis to resume with concurrent translocation into or insertion across the membrane. A SRP54 antibody supports studies focused on protein targeting, translation regulation, and secretory pathway biology.

SRP54 is ubiquitously expressed across tissues and cell types, reflecting the universal requirement for accurate targeting of secreted and membrane proteins. Cells with high secretory activity, such as plasma cells and glandular epithelial cells, rely heavily on efficient SRP function to maintain protein homeostasis. SRP54 interacts with SRP RNA and other SRP subunits to form a functional complex, and these interactions are tightly regulated to ensure fidelity and efficiency of protein targeting during translation.

From a disease-relevance perspective, alterations in SRP54 function have been linked to defects in protein secretion and cellular stress responses. Mutations in SRP54 have been associated with congenital neutropenia and related hematological disorders, highlighting the importance of proper co-translational targeting for immune cell development and function. Disruption of SRP-mediated trafficking can also contribute to endoplasmic reticulum stress and impaired protein folding, processes that are implicated in a wide range of pathological conditions. These findings underscore the relevance of SRP54 in studies of protein biogenesis and disease-associated trafficking defects.

At the molecular level, Signal recognition particle 54 kDa protein contains conserved domains required for signal peptide recognition and GTP binding, enabling its role as a regulatory switch during protein targeting. Post-translational modifications and dynamic interactions with ribosomes and membranes can influence its behavior in biochemical assays and SDS-PAGE without implying changes in primary sequence. A SRP54 antibody supports research applications focused on translational control, secretory pathway regulation, and disease-associated disruptions of protein targeting, with NSJ Bioreagents providing reagents intended for research use.

UniProt: P61011

Pathways: SARS-CoV-2 Protein Interaktom