Rekombinanter S100A12 Antikörper (AA 1-95)

Produktnummer ABIN8093448

Der Maus Monoklonal anti-S100A12 Antikörper (Klon MSVA-812M) (ABIN8093448) detektiert spezifisch S100A12 in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter S100A12 Antikörper (AA 1-95) (ABIN8093448)

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A12 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-812M

Produktdetails anti-S100A12 Antikörper

Bindungsspezifität: AA 1-95

Verwendungszweck: S100A12 Antibody / Calgranulin C / EN-RAGE

Immunogen: A recombinant fragment (around amino acids 1-95) of human S100A12 protein (exact sequence is proprietary) was used as the immunogen for the S100A12/S100 calcium binding protein A12 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the S100A12 Antibody for IHC / S100 Calcium Binding Protein A12 Immunohistochemistry Antibody should be determined by the researcher.

2. This S100A12/S100 calcium binding protein A12 antibody is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: S100A12/S100 calcium binding protein A12 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Andere Bezeichnung: S100A12

Hintergrund: S100 calcium binding protein A12 (S100A12) is a calcium-binding protein encoded by the S100A12 gene and belonging to the S100 family of EF-hand calcium-binding proteins. S100A12 Antibody for IHC enables visualization of S100 Calcium Binding Protein A12 expression in formalin-fixed paraffin-embedded tissue sections using immunohistochemistry. S100A12, also referred to as Calgranulin C or EN-RAGE (extracellular newly identified RAGE-binding protein), is predominantly expressed by neutrophils and activated myeloid cells where it functions as a pro-inflammatory mediator involved in innate immune responses and inflammatory signaling pathways.

Immunohistochemistry studies commonly detect S100A12 protein within neutrophils, granulocytes, and other inflammatory cell populations present in tissues undergoing inflammatory responses. In histological sections, S100A12 staining typically appears within the cytoplasm of infiltrating neutrophils and inflammatory cells, allowing clear visualization of these cells within the surrounding tissue architecture. Because S100A12 is associated with inflammatory cell infiltration, immunohistochemical staining is often used to evaluate inflammatory microenvironments and immune cell presence within tissue samples.

Large-scale tissue expression analysis using human tissue microarray (TMA) panels provides an efficient method for examining S100A12 distribution across many tissue types simultaneously. In human tissue microarray studies containing multiple normal and cancer tissues, S100A12 staining is generally restricted to scattered inflammatory cells rather than epithelial or stromal compartments. These patterns allow investigators to distinguish inflammatory cell populations within different tissues and to evaluate immune infiltration across a broad spectrum of tissue environments while maintaining consistent staining conditions across all specimens.

A recombinant mouse monoclonal antibody, clone MSVA-812M, recognizes S100A12 protein and supports immunohistochemical detection of S100A12 in FFPE human tissues. When applied to human tissue microarray (TMA) panels containing multiple normal and cancer tissues, S100A12 Antibody for IHC highlights neutrophils and other myeloid-derived inflammatory cells present within tissue sections. These staining patterns allow researchers to visualize inflammatory cell localization directly within histological tissue architecture and facilitate studies examining immune cell distribution and inflammatory responses in normal and disease-associated tissues.

This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteine