Rekombinanter FTO Antikörper

Produktnummer ABIN8093172

Der Kaninchen Monoklonal anti-FTO Antikörper (Klon AEAA-6) (ABIN8093172) detektiert spezifisch FTO in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für Rekombinanter FTO Antikörper (ABIN8093172)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser FTO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: AEAA-6

Produktdetails anti-FTO Antikörper

Verwendungszweck: FTO Antibody / Fat mass and obesity associated protein

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human Fat mass and obesity associated protein was used as the immunogen for the FTO antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the FTO antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the FTO antibody at -20oC.

Details zu FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Andere Bezeichnung: FTO

Hintergrund: FTO antibody targets Fat mass and obesity associated protein (FTO), a nuclear protein that functions as an RNA demethylase and plays an important role in post-transcriptional regulation of gene expression. FTO belongs to the AlkB family of dioxygenases and is primarily localized to the nucleus, where it associates with chromatin and RNA-processing machinery. Through its enzymatic activity, FTO removes methyl groups from specific RNA modifications, linking cellular metabolism to regulation of RNA stability, splicing, and translation. This positioning makes FTO a key molecular interface between nutrient status and gene expression control.

Functionally, FTO is best known for its ability to demethylate N6-methyladenosine (m6A) and related RNA modifications, which are critical regulators of RNA fate. By modulating RNA methylation status, FTO influences transcript processing, turnover, and translational efficiency. These effects impact diverse cellular processes, including energy balance, cell growth, and differentiation. A FTO antibody supports studies focused on RNA epigenetics and the mechanisms by which RNA modifications regulate gene expression programs.

FTO is broadly expressed across tissues, with notable expression in the brain, particularly in regions involved in energy homeostasis and feeding behavior. Its expression pattern reflects roles in both central nervous system regulation and peripheral metabolic tissues. Analysis of FTO localization and abundance provides insight into how RNA methylation dynamics respond to physiological cues such as nutrient availability and hormonal signaling. Because FTO activity is sensitive to cellular metabolic state, it is frequently examined in studies linking metabolism to gene regulatory mechanisms.

From a biological and disease-relevance perspective, FTO has been extensively studied in the context of metabolic regulation and human disease. Genetic variation in the FTO locus has been strongly associated with obesity risk and body mass index in population studies, driving significant interest in its biological function. Beyond metabolism, FTO has also been implicated in cancer biology, neural development, and stress responses, where altered RNA methylation patterns can influence cell behavior. Investigating FTO expression and regulation contributes to understanding how RNA modification pathways participate in disease-associated phenotypes.

At the molecular level, FTO is encoded by the FTO gene and produces a protein of approximately 58 kDa. The protein contains conserved catalytic motifs required for iron- and alpha-ketoglutarate-dependent demethylase activity. FTO function depends on proper nuclear localization and interaction with RNA substrates and regulatory cofactors. A FTO antibody supports research applications focused on RNA modification, metabolic signaling, and disease-related changes in gene regulation, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q9C0B1