TDP2 Antikörper (AA 201-300)

Produktnummer ABIN755782

Produktdetails anti-TDP2 Antikörper

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Bindungsspezifität: AA 201-300

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Maus

Homologie: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ETS1 associated protein II/EAPII

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu TDP2

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Andere Bezeichnung: ETS1 associated protein 2 (TDP2 Produkte)

Synonyme: AD022 antikoerper, EAP2 antikoerper, EAPII antikoerper, TTRAP antikoerper, dJ30M3.3 antikoerper, hTDP2 antikoerper, ad022 antikoerper, eap2 antikoerper, ttrap antikoerper, D13Ertd656e antikoerper, Ttrap antikoerper, tdp2 antikoerper, ttrapl antikoerper, tyrosyl-DNA phosphodiesterase 2 antikoerper, tyrosyl-DNA phosphodiesterase 2 S homeolog antikoerper, tyrosyl-DNA phosphodiesterase 2b antikoerper, TDP2 antikoerper, tdp2.S antikoerper, Tdp2 antikoerper, tdp2b antikoerper

Hintergrund:

Synonyms: EAP 2, EAP II, EAP2, EAPII, ETS 1 associated protein 2, ETS 1 associated protein II, ETS1 associated protein 2, ETS1-associated protein 2, ETS1-associated protein II, tdp2, TRAF and TNF receptor associated protein, TRAF and TNF receptor-associated protein, TTRAP, TYDP2_HUMAN, Tyr DNA phosphodiesterase 2, Tyr-DNA phosphodiesterase 2, Tyrosyl DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2, 5''-Tyr-DNA phosphodiesterase, 5''-tyrosyl-DNA phosphodiesterase, AD 022, AD022, MGC111021, MGC9099.

Background: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.