Shugoshin Antikörper (N-Term)

Produktnummer ABIN8037120

Der Kaninchen Polyklonal anti-Shugoshin Antikörper wird verwendet zum Nachweis von Shugoshin in Proben von Human und Maus. Er wurde validiert für WB, IHC und FACS.

Kurzübersicht für Shugoshin Antikörper (N-Term) (ABIN8037120)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Shugoshin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-Shugoshin Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-SGOL1/SGO1 Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SGOL1/SGO1 Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SGOL1/SGO1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Andere Bezeichnung: SGO1

Hintergrund:

Background: The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Gene Full Name: shugoshin 1

Molekulargewicht: 75 kDa

Gen-ID: 151648

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location