ECT2 Antikörper (AA 781-883)

Produktnummer ABIN753853

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ECT2 in WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für ECT2 Antikörper (AA 781-883) (ABIN753853)

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ECT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ECT2 Antikörper

Bindungsspezifität: AA 781-883

Kreuzreaktivität: Human, Ratte

Homologie: Mouse,Dog,Cow,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ECT2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-ECT2 Antikörper (ABIN753853)

Wang, Yan, Liu: "Clinical significance of ECT2 expression in tissue and serum of gastric cancer patients." in: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, Vol. 18, Issue 7, pp. 735-42, (2016) (PubMed).

Guo, Chen, Du, Zhu, Lai, Dong, Wu, Lin, Liu, Huang: "Elevated levels of epithelial cell transforming sequence 2 predicts poor prognosis for prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 34, Issue 1, pp. 13, (2016) (PubMed).

Details zu ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Andere Bezeichnung: ECT2

Hintergrund:

Synonyms: ARHGEF31, Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2

Background: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly, regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity, participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Gen-ID: 1894

UniProt: Q9H8V3

Pathways: Neurotrophin Signalübertragung, Cell-Cell Junction Organization