PYGL Antikörper (AA 313-804)

Produktnummer ABIN8022433

Der Kaninchen Polyklonal anti-PYGL Antikörper (ABIN8022433) detektiert spezifisch PYGL in WB, IHC, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für PYGL Antikörper (AA 313-804) (ABIN8022433)

Target: PYGL (phosphorylase, Glycogen, Liver (PYGL))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PYGL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-PYGL Antikörper

Bindungsspezifität: AA 313-804

Verwendungszweck: Anti-PYGL Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PYGL Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PYGL recombinant protein (Position: K313-K804).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PYGL

Target: PYGL (phosphorylase, Glycogen, Liver (PYGL))

Andere Bezeichnung: PYGL

Hintergrund:

Background: Glycogen phosphorylase, liver form (PYGL), also known as human liver glycogen phosphorylase (HLGP), is an enzyme that in humans is encoded by the PYGL gene on chromosome 14. This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: glycogen phosphorylase L

Molekulargewicht: 97 kDa

Gen-ID: 5836

UniProt: P06737

Pathways: Carbohydrate Homeostasis, Cellular Glucan Metabolic Process