PEMT Antikörper (C-Term)

Produktnummer ABIN8021232

Der Kaninchen Polyklonal anti-PEMT Antikörper wird verwendet zum Nachweis von PEMT in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und FACS.

Kurzübersicht für PEMT Antikörper (C-Term) (ABIN8021232)

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PEMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-PEMT Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-PEMT Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human PEMT shares 94.7%,84.2% amino acid (aa) sequence identity with mouse,rat PEMT,respectively.

Produktmerkmale: Anti-PEMT Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PEMT. Human PEMT shares 94.7% and 84.2% amino acid (aa) sequence identity with mouse and rat PEMT, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PEMT

Target: PEMT (Phosphatidylethanolamine N-Methyltransferase (PEMT))

Andere Bezeichnung: PEMT

Hintergrund:

Background: Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Gene Full Name: phosphatidylethanolamine N-methyltransferase

Molekulargewicht: 17 kDa

Gen-ID: 10400

UniProt: Q9UBM1