Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) Antikörper (HRP)

Produktnummer ABIN8010801

Der Kaninchen Polyklonal anti-Mitochondrially Encoded NADH Dehydrogenase 4 Antikörper wird verwendet zum Nachweis von Mitochondrially Encoded NADH Dehydrogenase 4 in Proben von Human. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) Antikörper (HRP) (ABIN8010801)

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: AA 10-459

Verwendungszweck: Anti-MT-ND4 Antibody HRP Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Andere Bezeichnung: MT-ND4

Hintergrund:

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Gene Full Name: NADH dehydrogenase subunit 4

Gen-ID: 4538

UniProt: P03905